Purification and properties of the ribonucleic acid-dependent ribonucleic acid polymerase from Halobacterium cutirubrum.

HALOBACTERIUM CUTIRUBRUM DEOXYRIBONUCLEIC ACID-DEPENDENT RIBONUCLEIC ACID POLYMERASE By B. GREGORY LOUIS AND P. S. FITT

1. DNA-dependent RNA polymerase was purified 150-fold from crude extracts of the extreme halophile Halobacterium cutirubrum. 2. The enzyme requires the presence of native DNA and all four nucleoside triphosphates to incorporate '4C-labelled nucleoside triphosphate into an acid-insoluble ribonuclease-sensitive product. 3. It has an absolute requirement for both Mn2+ and Mg2+. 4. The polymerase r...

The role of deoxyribonucleic acid in ribonucleic acid synthesis. I. The purification and properties of ribonucleic acid polymerase.

It has become evident that several enzymatic reactions exist which lead to the incorporation of the mononucleotide moiety of the ribonucleoside triphosphates into ribonucleic acid (1). The first of these enzymes to be characterized catalyzes the addition of cytosine 5’-phosphate and adenosine 5’-phosphate to the nucleoside terminal end of soluble ribonucleic acid (2,3). The product of this reac...

Indirect evidence for the presence of Ribonucleic acid in vesicular stomatitis Virions

The influence of syringomycin on ribonucleic acid synthesis.

Syringomycin, a wide-spectrum antibiotic produced by strains of Pseudomonas syringae which cause bacterial canker of peach, was able to bind to salmon sperm and calf thymus deoxyribonucleic acid but not to calf thymus histone; it also inhibited ribonucleic acid polymerase activity. These abilities to bind to deoxyribonucleic acid and to inhibit ribonucleic acid polymerase were inactivated when ...

Gel-filtration and Density-gradient-centrifugation Studies of the Molecular Weights of Halobacterium Cutirubrum

1. Conditions have been established for the estimation of molecular weights of proteins by analytical gel filtration and sucrose-density-gradient centrifugation in 2.5M-potassium chloride-l M-sodium chloride; Halobacterium cutirubrum polynucleotide phosphorylase, DNA-dependent RNA polymerase and RNA-dependent RNA polymerase have been studied by these methods. 2. The RNA-dependent polymerase has...